Development and characterization of microsatellite loci for three Ocotea species (Lauraceae) threatened with extinction

MARTINS EM; MARTINELLI G; ARBETMAN MP; LAMONT RW; SIMÕES-ARAÚJO JL; POWELL D; CIAMPI-GUILLARDI M; BALDAUF C; QUINET A; GALISA P; SHAPCOTT A

GENETICS AND MOLECULAR RESEARCH

FUNPEC-EDITORA

Año: 2014 vol. 13 p. 5138 - 5142

1676-5680

The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as  threatened due to overexploitation. To investigate the genetic diversityand population structure of these species, we developed 8 polymorphicmicrosatellite markers for O. odorifera from an enriched microsatellitelibrary by using 2 dinucleotide repeats. The microsatellite markerswere tested for cross-amplification in O. catharinensis and O. porosa.The average number of alleles per locus was 10.2, considering allloci over 2 populations of O. odorifera. Observed and expectedheterozygosities for O. odorifera ranged from 0.39 to 0.93 and 0.41to 0.92 across populations, respectively. Cross-amplification of all lociwas successfully observed in O. catharinensis and O. porosa except 1locus that was found to lack polymorphism in O. porosa. Combinedprobabilities of identity in the studied Ocotea species were very lowranging from 1.0 x 10-24 to 7.7 x 10-24. The probability of exclusion overall loci estimated for O. odorifera indicated a 99.9% chance of correctlyexcluding a random nonparent individual. The microsatellite markersdescribed in this study have high information content and will be usefulfor further investigations on genetic diversity