Platelets directly interact with Brucella abortus and modulate infected human monocytes towards a pro-inflammatory profile

TROTTA, A; MILILLO, MA; DELPINO, MV; VELÁSQUEZ, LN; RODRÍGUEZ, AM; GIAMBARTOLOMEI, GH; POZNER, RG; BARRIONUEVO, P

Buenos Aires

Congreso; LXII Reunión Anual de SAIC. LIII Reunión Anual de SAIB. LXV Reunión Anual de SAI. Reunión Anual de SAA. XLVI Reunión Anual de SAB. XIX Reunión Anual de SAB. XLIX Reunión Anual de SAFE. Reunión Anual de SAFIS. Reunión Anual de SAH. XXIX Reunión Anual de SA; 2017

Brucellosis is an infectious disease elicited by bacteria of the genus Brucella. Platelets have been described as mediators of hemostasis. However, they have recently got involved in the modulation of innate and adaptive immune responses. We have previously reported that platelets modulate B. abortus-mediated infection of human monocytes. The first aim of this study was to characterize the interaction between platelets and B. abortus. For this, human platelets were co-incubated with B. abortus-GFP for 4 h and the interaction was quantified by confocal microscopy and flow cytometry. Our results showed that platelets are able to interact with bacteria in a dose-dependent fashion. Moreover, this interaction stimulated platelet activation, measured as fibrinogen binding and P-selectin expression (p