Brucella abortus-stimulated platelets actívate brain microvascular endotelial cells

RODRÍGUEZ, AM; TROTTA, A; MIRAGLIA, MC; DELPINO, MV; BARRIONUEVO, P; GIAMBARTOLOMEI, GH

Buenos Aires

Congreso; LXII Reunión Anual de SAIC. LIII Reunión Anual de SAIB. LXV Reunión Anual de SAI. Reunión Anual de SAA. XLVI Reunión Anual de SAB. XIX Reunión Anual de SAB. XLIX Reunión Anual de SAFE. Reunión Anual de SAFIS. Reunión Anual de SAH. XXIX Reunión Anual de SA; 2017

Central nervous system invasion by bacteria of the genus Brucella results in an inflammatory disorder called neurobrucellosis. Blood-brain barrier activation is a common feature of human neurobrucellosis, but the underlying mechanisms are largely unknown. The aim of this work was to investigate the influence of B. abortus (B.a.)-activated platelets (PTL) on human brain microvascular endothelial cells (HBMEC). For this, supernatants (SN) from B.a.-stimulated human PTL were used to stimulate HBMEC. Expression of ICAM-1 (CD54) and secretion of IL-6, IL-8 and MCP-1 were evaluated to determine HBMEC´ activation. SN from B.a.-stimulated PLT induced a significant secretion of IL-6, IL-8, MCP-1 and the up-regulation of CD54 expression in a dose-dependent manner compared with SN from unstimulated PLT (p0.05), demonstrating that a soluble factor released from PLT was implicated on HBMEC? activation. SN from heat-killed B.a.-stimulated PTL also activated HBMEC indicating that B.a.? viability was not necessary in activating PLT. Soluble CD40L was not implicated on HBMEC? activation, as an anti-CD40L antibody could not block cytokine secretion or ICAM-1 up-regulation. Treatment of SN with trypsin eliminate the effect (p