Immune-related gene expression in honey bee larva (Apis mellifera) exposed to plant extracts from Humulus lupulus with antimicrobial activity against Paenibacillus larvae

GIMÉNEZ MARTÍNEZ, PABLO DARIAN; ZUÑIGA FABIAN; JUNGES CELINA; MAGGI MATIAS; FUSELLI SANDRA; MARTINEZ JESSICA

APIDOLOGIE

EDP SCIENCES S A

Lugar: Paris; Año: 2021

0044-8435

American foulbrood caused by Paenibacillus larvae, brings with it several problems on beekeepers for the use of synthetic antibiotics, like wise led to the appearance of resistant strains and the presence of chemical residues in the commercial products of the hive. In recent years, the use of natural compounds for the control of P. larvae has increased. Herein, we propose to determine the value of the Minimal Inhibitory Concentration (MIC) of a hydroalcoholic extract of Humulus lupulus (hop) leaves of Victoria variety on P. larvae strain, and to evaluate its toxicity and effect on the expression of three genes of immune system on honey bee larvae. For the extract, the MIC value was 83.79 µg/mL. For larvae the extract didn´t show toxicity, on the other hand presented a down-regulation in the gene expression for the three genes evaluated. The results obtained here are a first report on the evaluation in the effect of natural extracts on the expression of genes related to the immune system in bee larvae.