BECAS
BETANCOURT GORDILLO Diana Marcela
congresos y reuniones científicas
Título:
Foodborne diseases by Salmonella enterica serovar Enteritidis during early stages of puerperium induce an adverse maternal outcome in the murine model.
Autor/es:
NOTO LLANA M; KRUMMEL L; GARÓFALO A; MEISS R; BETANCOURT D; SARNACKI S; BUZZOLA F; CERQUETTI M; GIACOMODONATO M
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias. LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica SAIC 2017; 2017
Institución organizadora:
SAIC
Resumen:
Diarrheal disease agents, especially non-typhoidal Salmonella enterica (S. Enteritidis), are responsible for the majority of deaths due to foodborne diseases (FBD). Puerperium is a predisposing condition for exacerbation of infections. Our aim was to study the effect of FBD by S. Enteritidis during the early puerperium. To this purpose, we used an enterocolitis model. BALB/c mice received 20 mg of streptomycin 48 hs postpartum. 24 hs later they received 3,500 CFU of S. Enteritidis intragastrically (sub lethal dose). At day 3 post infection (day 6 postpartum) intestinal permeability was measured in vivo. Gut samples were analyzed for bacterial colonization, histological changes and tight junctions mRNA levels. Ileum and serum citoquines expression were studied by qPCR and ELISA, respectively. We found that 86% of puerperal females died within 5 days post infection whereas the mortality rate of infected virgin mice was 20%.This adverse maternal outcome is correlated with massive bacterial organs colonization. Also, gut permeability was significantly increased in infected parturient females (p