CANOSA Ivana SofÍa
congresos y reuniones científicas
Histopatological effects of the herbicide atrazine on ovary and hepatopancreas of the estuarine crab Neohelice granulata
SILVEYRA, GABRIELA R.; IVANA S. CANOSA; MAC LOUGHLIN, CAMILA; MEDESANI, DANIEL A.; RODRÍGUEZ, ENRIQUE M.
Ciudad de Buenos Aires
Congreso; 11th SETAC Latin America Biennial Meeting "The role of science in environmental decision-making"; 2015
Adult females (10.86 ± 0.08 g) of the estuarine crab Neohelice granulata were exposed for 12 wk (June to September) to Gesaprim90®, at 0.03, 0.3 and 3 mg/L of atrazine as active principle in the commercial formulation. A control group with no atrazine added was also run. Fifteen animals were randomly assigned to each treatment. Each female was isolated in a glass recipient filled with 400 mL of artificial saline water, at 12 g/L, under continuous aeration and controlled conditions of temperature (22 ºC) and photoperiod (14:10 L:D). During the assay, animals were fed three times a week with food pellets and fresh leaves of Elodea sp., renewing all test solutions twice a week. At the end of the assay, the ovary and hepatopancreas of all females were dissected, weighed and fixed in Bouin solution for histological processing. Females exposed to the highest concentration of atrazine showed a gonadosomatic index 50% lower than that of control females. Histological analysis indicated that females exposed to the same atrazine concentration had a reduced proportion of vitellogenic oocytes with respect to control, together with a significantly (p< 0.05) higher proportion of reabsorbed oocytes. No differences were observed among treatments concerning the hepatopancreatic index. The analysis of hepatopancreatic cell types indicated a significant (p< 0.05) reduction in the proportion of F-cells, in females exposed to 5 mg/L of atrazine. These results suggest an inhibition of ovarian growth by atrazine as a possible endocrine disrupter, in accordance with previous studies recently published.