BARRAZA Daniela EstefanÍa
Expression of β-ngf and high affinity ngf receptor (trka) in llama ( Lama Glama ) male reproductive tract and spermatozoa
SARI, LUCIANA M.; ZAMPINI, RENATO; ARGAÑARAZ, MARTIN E.; CARRETERO, MARÍA I.; FUMUSO, FERNANDA G.; BARRAZA, DANIELA E.; RATTO, MARCELO; APICHELA, SILVANA A.
MOLECULAR REPRODUCTION AND DEVELOPMENT
WILEY-LISS, DIV JOHN WILEY & SONS INC
Lugar: New York; Año: 2018
β-Nerve Growth Factor (β-NGF) is a seminal plasma element, responsible for inducing ovulation in camelids. The main organ of β-NGF production remains nondescript. The aims of this study were: (1) characterize gene expression and protein localization of β-NGF and its main receptor TrKA in the llama male reproductive tract, and (2) determine whether the seminal β-NGF interacts with ejaculated sperm by localizing β-NGF and TrKA in epididymal, ejaculated, and acrosome reacted (AR) sperms and, additionally, by identifying β-NGF presence in sperm-adsorbed proteins (SAP). Both β-NGF and TrkA transcripts are widely expressed along the male reproductive tract, with a higher expression level of β-NGF at prostate (p