congresos y reuniones científicas
HEMOLYSIS AND ANTIHEMOLYSIS INDUCED BY ARGININE-BASED SURFACTANTS
HERMET, M.; FAIT, M.E.; COMELLES, F.; CLAPÉS. P.; ÁLVAREZ, A.; PRIETO, E.; VÁZQUEZ, R.; MATÉ, S.; HERLAX, V.; MORCELLE, S.R.; BAKÁS, L.
Congreso; Reunión Conjunta de Sociedades de Biociencas; 2017
Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized in terms of surface properties and interaction with human red blood cells (HRBC) membranes. CMC values, Γmax (maximum surfactant adsorption at the air/liquid interface) and Amin (area per molecule) revealed better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn through atomic force microscopy (AFM) supported the predictions based on the surfactant packing parameter value (SPP). Erythrocyte membrane solubilization was induced by surfactant aggregates, since cell lysis was only evidenced at surfactant concentrations above CMC. Changes in HRBC shape observed at different surfactant concentrations allowed to conclude that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by shedding of microvesicles is responsible for the hemolysis produced by both surfactants at the lower concentrations tested. On the other hand, the extraction of membrane lipids upon collisions between HRBC and surfactant aggregates competes and prevents the microvesicles release for the higher concentrations assayed. Moreover, we study the interaction of Bz-Arg-NHC12 with sheep red blood cells (SRBC) and HRBC due to their different membrane protein/lipid composition. SRBC is a little more resistant than HRBC to the hemolytic effect of surfactant, but in both cases, the micellar form of the surfactant is the entity responsible of the hemolytic effect. As HC50, cAHmax value was higher than the CMC value. Thus, a biphasic behavior was observed for the surfactant studied, showing a wide range of protective concentrations when HRBC were tested, while for SRBC, the degree of protection of Bz-Arg-NHC12 was about 50% lower than for HBRC. However, only for SRBC treated with Bz-Arg-NHC12 a remarkable volume expansion was evidence, although no correlation with the antihemolytic potency was found.