INVESTIGADORES
ROMANO Nelson GastÓn
congresos y reuniones científicas
Título:
Scale up of layer-by-layer microencapsulation of probiotic bacteria Lactiplantibacillus plantarum WCFS1
Autor/es:
GINA TAVARES; NELSON ROMANO; MARIA GUERRERO-SANCHEZ; MARINA GOLOWCZYC; STEPHANIE PASSOT; SONIA CAMPOY; FERNANDA FONSECA; PEDRO SIMOES; ANDREA GÓMEZ-ZAVAGLIA
Lugar:
Cambridge
Reunión:
Exposicin; WORKSHOP - Preservation of microorganisms by understanding the protective mechanisms of oligosaccharides; 2023
Institución organizadora:
INRA -Cytiva
Resumen:
The layer-by-layer (LbL) microencapsulation of bacteria is an emerging process that will make it possible to develop more eco-friendly pocesses for lactic acid bacteria. This method takes advantage of the sequential deposition of oppositely charged polymers driven by electrostatic interactions to form nanoscale thin films on planar or colloidal surfaces. However, LbL is typically time-consuming, and automation of the polymer deposition steps is difficult to scale up. In this regard, this study aims to optimize the scale-up of LbL microencapsulation of Lactiplantibacillus plantarum WCFS1. Chitosan and alginate were used as cationic and anionic polyelectrolytes, respectively. Processing and formulation parameters like biomass production, washing steps, number of polymer layers and biomass/polymer ratio during the encapsulation were evaluated. The coating process was monitored by ?-potential measurements. Numeration of culturable cells was performed before and after coating, after spray-drying with fructo-oligosaccharides and maltodextrin as the protective matrix and after exposure to simulated gastrointestinal conditions.