INVESTIGADORES
LUJAN Adela Maria
congresos y reuniones científicas
Título:
Hypermutability and adaptive phenotypes in cystic fibrosis isolates of Pseudomonas aeruginosa
Autor/es:
FELIZIANI, SOFIA; LUJAN, ADELA M; MOYANO, ALEJANDRO J; SOLA, CLAUDIA; BOCCO, JOSE LUIS; MONTANARO PRATRICIA; FERNANDEZ CANIGGIA, LILIANA; ARGARAÑA CARLOS E; SMANIA, ANDREA M
Lugar:
Carlos Paz, Cordoba
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2008
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
During Cystic Fibrosis (CF) pulmonary chronic infection, P.aeruginosa diversifies into phenotypes with particular traits -such asmucoids, avirulent, hypermutators and multiresitant antibioticvariants- which favor P. aeruginosa long-term persistence andprevent its eradication. The acquisition of these phenotypes mostlyinvolves mutations in target genes which would be catalyzed byhypermutators. Indeed, the link between antimicrobial resistanceand hypermutators was suggested. Also, we established a linkbetween hypermutability and emergence of mucoid and avirulentvariants in vitro. In order to study the role of hypermutability in P.aeruginosa phenotypic diversification in vivo, we analyzed theassociation between hypermutator phenotype and mucoid, avirulentand multi drug resistant variants (mucA, lasR, mexZ mutantsrespectively) in 40 P. aeruginosa isolates from 27 CF patients. Weobserved that 20 isolates (50%) were hypermutators and 14 patients(55.5%) had hypermutators. Regarding mucA, lasR and mexZ, twoisolates (5%) showed unaltered sequences in the three genes and anon mutator phenotype. Also, 75% of the isolates showed mutationsin mexZ of which 40% were hypermutators. In addition, 65% ofisolates showed mutations in mucA, of which 46% werehypermutators. Finally, a lower percentage of isolates (37%)harboured mutations in lasR, however, 66% of them werehypermutators.