INVESTIGADORES
TASSO Mariana Patricia
congresos y reuniones científicas
Título:
NanoPaint: tracking of the cannabinoid type 1 receptor with biofunctional quantum dot nanoconstructs reveals fast nanoscopic structural plasticity of the neuronal cell membrane
Autor/es:
ZALA, DIANA; PONS, THOMAS; LEQUEUX, NICOLAS; LENKEI, ZSOLT; TASSO, MARIANA
Lugar:
San Diego
Reunión:
Conferencia; Neuroscience 2018; 2018
Institución organizadora:
Society for Neuroscience
Resumen:
Single-particle tracking with quantum dots constitutes a powerful tool to follow the dynamics of cell membrane receptors unveiling their intricatemechanisms of membrane diffusion, velocity, endocytic uptake and fate. Super-resolution microscopy and live imaging in three dimensions (3D) ofcell membrane-bound quantum dots (QDs) also provide a wealth of single-particle trajectories that can be exploited as a source of spatialinformation to effectively reconstruct the cell membrane in 3D with high spatio-temporal resolution. Single QD nanoparticles tracking cell receptordynamics can accurately and rapidly (seconds) ?paint? the cell membrane with a level of 3D detail that not only sets the ground to analyze cellmembrane deformation under pertinent biological stimuli, but also sheds light on the membrane topography and membrane plasticity. In this work,very bright, small and stable biofunctional QD nanoconstructs were employed to demonstrate the "nanoPaint" concept in conjunction with live 3Dsuper-resolution microscopy. QDs recognizing the neuronal cannabinoid type 1 receptor (CB1R) were used to ?paint? the cell membrane and to follow membrane deformations of mature cultured rat hippocampal neurons. We show that the high axonal expression of CB1R and its rapid membrane diffusion are interesting characteristics to fully reconstruct pre-synaptic terminals including the plasma-membrane juxtaposing thesynaptic cleft. Typically, less than one minute acquisition is sufficient to obtain enough information for a 3D reconstruction with a resolution in the tens of nanometers range. Time-lapse reconstructions at 30s to 1min temporal resolution of filopodia and axons highlights the high nanoscale dynamics of the cell membrane shape. Insights of QD penetration in the neuronal synaptic cleft highlight the potential of nanoPaint method as a precision tool for neuronal structural plasticity studies.