INVESTIGADORES
TASSO Mariana Patricia
congresos y reuniones científicas
Título:
QUANTUM DOT-BASED CLUSTERS FUNCTIONALIZED FOR BIODETECTION
Autor/es:
DEMBELE, FATIMATA; TASSO, MARIANA; FRAGOLA, ALEXANDRA; LORIETTE, VINCENT; LEQUEUX, NICOLAS; PONS, THOMAS
Lugar:
Lisbon
Reunión:
Conferencia; 5th International Conference on Multifunctional, Hybrid and Nanomaterials; 2017
Resumen:
Using nanotechnology for molecular diagnostics holds many advantagese.g. an improvement in the simplicity and the sensitivity of analysis. Quantumdot (QD) semi-conductor nanocrystals demonstrate several properties that makethem suitable probes for biomolecular recognition. These nanocrystals presentnarrow size-tunable emission spectra and a broad excitation spectrum; inaddition, they offer higher photostability and brightness than conventionalorganic dyes. The objective of this study is to develop a new diagnostic toolbased on fluorescent nanobeads containing QDs for fast and single-moleculedetection. Our strategy consists inself-assembling QDs into clusters of a few hundreds of nanometer diameter. Thisway, we can achieve an even brighter fluorescence and easily detectableanalytical signals as compared to single QDs. After the clusters are formed, asilica shell is grown on their surface by a Stöber-inspired process in order toprotect their colloidal and photo-stability. A new type of multidentatepolymer-silane hybrid is subsequently grafted onto the silica shell, presentinga zwitterionic chain for water solubility and reactive functions forconjugation with biomolecules. Our team has succeeded in reacting streptavidin-conjugatednanobeads with commercial biotinylated beads. Preliminary results have alsoshown that we can integrate the nanobeads into a microfluidic system for anefficient single-particle counting.