TASSO Mariana Patricia
Multimodal Mn-doped I-III-VI quantum dots for near infrared fluorescence and magnetic resonance imaging: From synthesis to in vivo application
SITBON, GARY; BOUCCARA, SOPHIE; TASSO, MARIANA; FRANCOIS, AURÉLIE; BEZDETNAYA, LINA; MARCHAL, FRÉDÉRIC; BEAUMONT, MARINE; PONS, THOMAS
Royal Society of Chemistry
Año: 2014 vol. 6 p. 9264 - 9272
The development of sensitive multimodal contrast agents is a key issue to provide better global, multi-scale images for diagnostic or therapeutic purposes. Here we present the synthesis of Zn-Cu-In-(S, Se)/Zn 1-xMnxS core-shell quantum dots (QDs) that can be used as markers for both near-infrared fluorescence imaging and magnetic resonance imaging (MRI). We first present the synthesis of Zn-Cu-In-(S, Se) cores coated with a thick ZnS shell doped with various proportions of Mn. Their emission wavelengths can be tuned over the NIR optical window suitable for deep tissue imaging. The incorporation of manganese ions (up to a few thousand ions per QD) confers them a paramagnetic character, as demonstrated by structural analysis and electron paramagnetic resonance spectroscopy. These QDs maintain their optical properties after transfer to water using ligand exchange. They exhibit T1-relaxivities up to 1400 mM-1 [QD] s-1 at 7 T and 300 K. We finally show that these QDs are suitable multimodal in vivo probes and demonstrate MRI and NIR fluorescence detection of regional lymph nodes in mice. This journal is © the Partner Organisations 2014.