INVESTIGADORES
TASSO Mariana Patricia
artículos
Título:
Covalent Immobilization of Subtilisin A onto Thin Films of Maleic Anhydride Copolymers
Autor/es:
MARIANA TASSO; ANA CORDEIRO; KATRIN SALCHERT; CARSTEN WERNER
Revista:
MACROMOLECULAR BIOSCIENCE
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2009 vol. 9 p. 922 - 929
ISSN:
1616-5187
Resumen:
Enzymes cleaving the biopolymer adhesives of fouling organisms are attracting attention forthe prevention of biofouling. We report a versatile and robust method to confine the serineprotease Subtilisin A (or Subtilisin Carlsberg) to surfaces to be protected against biofouling.The approach consists of the covalent immobilization of the protease onto maleic anhydridecopolymer thin film coatings. High-swelling poly(ethylene-alt-maleic anhydride) (PEMA) copolymer layers permitted significantly higher enzyme loadings and activities than compact poly(octadecene-alt-maleic anhydride) (POMA) films. Substantial fractions of the immobilized, active enzyme layers were found to be conserved upon storage in deionized water for several hours. Ongoing studies explore the potentialities of the developed bioactive coatings to reduce the adhesion of various fouling organisms.