Puesta a punto de una técnica de identificación de Plasmodium (Plasmodiidae) agente causal de la malaria mediante PCR múltiple semianidada a partir de muestras sanguíneas

STEIN MARINA; JOSE LUIS SILVA; RAÚL HORACIO LUCERO; LAURA FORMICELLI

Revista Bioquímica y Patología Clinica

Editorial de la Revista Bioquímica y Patplogía Clínica

Lugar: Buenos Aires; Año: 2013 vol. 77 p. 31 - 33

1515 6761

Currently in Argentina, are used very laborious microscopy techniques in diagnosing malaria, from blood samples. It is necessary to develop sensitive and specific techniques to facilitate such studies. With that goal, a technique of semi-nested multiplex PCR to detect Plasmodium DNA in blood samples and identify the parasite species was adapted. For this purpose, from blood samples on filter paper of malaria patients and from a culture of P. falciparum, DNA was extracted. Specific primers were used to amplify variable sequences present in the small subunit ribosomal (ssrRNA) from parasite, to detect the presence of DNA Plasmodium in the samples analyzed and identifying the parasite species present in them.