PERSONAL DE APOYO
KELLER Leticia
congresos y reuniones científicas
Título:
Diagnostic and strain identification of Canine Parvovirus in Argentina by Molecular Biology tools
Autor/es:
GALLO CALDERÓN, MARINA; KELLER, L; MARCELA IGLESIAS; VIVIANA MALIRAT; PERIOLO, OSVALDO; MATTION, NORA; LA TORRE, JOSÉ
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Canine Parvovirus (CPV) is a very contagious and fatal viral disease. Although live attenuated vaccines are available, viral evolution leads to several episodes of CPV throughout the world even in vaccinated animals. The original strain CPV2 was replaced in dog populations by two antigenic variants: CPV2a and CPV2b. Recently, a new antigenic variant carrying the aa substitution Asp426Glu, located at the major antigenic region, and called CPV2c, has spread all over the world. As diagnosis of CPV is difficult because clinical signs may be confused with other canine enteric diseases, the objective of the present work was to develop a specific diagnostic tool to detect and characterize CPV in clinical samples. A total of 75 samples were submitted to the laboratory. Samples were taken from domestic dogs between 2008 and 2010. Diagnosis was made by PCR amplification of a 583 bp fragment of VP2 gene which includes the 426 aminoacidic position and strain determination was performed by sequence analysis. CPV DNA was detected in 51 of 75 (68%) samples. 39 of the 51 positive clinical specimens (76%) were obtained from vaccinated dogs. Out of 51 positive samples, 46 (90%) belonged to the CPV-2c variant, 3 to CPV-2b, and the remaining 2 were CPV-2a. These results, suggest that the newest genotype CPV-2c has spread and replaced earlier variants becoming the predominant strain among dogs.