Designing a Highly Active Soluble PQQ-Glucose Dehydrogenase for Efficient Glucose Biosensors and Biofuel Cells

DURAND, F.; STINES-CHAUMEIL, C.; VICTORIA FLEXER; ANDRÉ, I.; MANO, N.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2010 vol. 402 p. 750 - 754

0006-291X

We report for the first time a soluble PQQ?glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the cofactor and the enzyme leading to a better electron transfer. Electrochemical experiments also demonstrate the superiority of the new mutant for glucose oxidation and make it a promising enzyme for the development of high-performance glucose biosensors and biofuel cells.