INVESTIGADORES
LAGORIO MarÍa Gabriela
informe técnico
Título:
Sentinel Plants for the Surveillance of Chemical Risk. Natural plant-based sentinel systems, to detect acetylcholinesterase (AChE) inhibiting pesticides (ACIPs) in the environment
Autor/es:
LAGORIO, MARÍA GABRIELA; GONZÁLEZ, GRACIELA; CORDON, GABRIELA B.; DIZ, VIRGINIA; PETRI, MAILÉN; LÓPEZ VALIÑO, IVANA; FILIPPINI, TOBÍAS RAMIRO; DIEGUEZ GAVIOLA, GONZALO; GONZÁLEZ BARDECI, NICOLÁS; GÜERCI, MICAELA; HUNT, ANDRÉS
Fecha inicio/fin:
2021-09-01/2023-12-31
Naturaleza de la

Producción Tecnológica:
Química
Campo de Aplicación:
Sanidad ambiental
Descripción:
This proposal plans to develop natural plant systems that will act as sentinels to the environmental presence of acetylcholinesterase inhibiting pesticides which are known to affect the function of the central nervous system.Cichorium intybus, Glycine max, Salvinia molesta, Vallisneria and Spatiphyllium wallisii were tested as potential sentinel plants for the presence of pesticides in the environment.Chlorpyrifos and dimethoate were used as pesticides for the experiments. Chlorpyrifos concentrations from 0.105 to 52.5 g/L were sprayed on Cichorium intybus and from 0.105 to 21.0 g/L on Glycine max. Additionally, dimethoate solutions with concentrations ranging from 0.375 to 7.5 g/L were sprayed on Cichorium intybus.Regarding the aquatic plants, the pesticide treatment was carried out by adding the pesticide to the aquatic media where the plant floated. In different experiments Chlorpyrifos, Dimethoate or a mixture Chlorpyrifos-Dimethoate were essayed.Pesticide concentrations ranged from 0.5 to 1000 ppm for experiments with the aquatic plants.Changes in spectroscopical and optical properties of plant leaves (fast transient of the variable chlorophyll-a fluorescence also called OJIP curve; Kautsky kinetics of variable chlorophyll-a fluorescence; spectral distribution of the initial chlorophyll-a fluorescence; reflectance and images) were analysed. The most sensitive parameters for the presence of pesticides were derived from the fast Chlorophyll-a fluorescence transient (OJIP Curve), which was recorded in vivo.In parallel, new methodologies for the determination of Acetylcholinesterase were developed.