Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of

ANALÍA IRIEL, M. GABRIELA LAGORIO

Cubatao. Brasil

Congreso; IX Encontro Latinoamericano de Fotoquímica y Fotobiologia; 2008

CEPEMA

Anthocyanins are phenolic compounds widely distributed in the vegetable kingdom. Due to their chemical structure and their acidic and basic properties, they have different colors and they are responsible for the appearance of many kinds of petals, fruits and of reddish leaves. Anthocyanins are relevant compounds in plant development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of chemical structure and their acidic and basic properties, they have different colors and they are responsible for the appearance of many kinds of petals, fruits and of reddish leaves. Anthocyanins are relevant compounds in plant development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of . Due to their chemical structure and their acidic and basic properties, they have different colors and they are responsible for the appearance of many kinds of petals, fruits and of reddish leaves. Anthocyanins are relevant compounds in plant development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of . Anthocyanins are relevant compounds in plant development (attracting insects for pollination and seed dispersion, acting as sunlight screener, etc) and in human health due to their valuable pharmacological applications as antioxidants. They are also significant as food colorants. Studies based on flowers spectroscopy are scarce in bibliography. Nevertheless, the characterization of their absorption, reflectance and fluorescence spectra, as well as the information on their scattering properties and the evaluation of their pigments concentration are significant in understanding development of attracting properties for pollinators. Additionally, anthocyanins content could be a bioindicator of a broader stress development within the natural environment of the species, and which could be assessed with near-remote methods. The scope of the present work is characterizing the optical properties and the fluorescence emission spectrum of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of Rhododendron indicum flowers and finding a correlation, between their anthocyanins concentration and the optical parameters, potentially useful in remote sensing. Reflectance spectra from pink petals of Rhododendron indicum flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water , in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in µmol.g-1 has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in µmol.g-1 has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water , in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in µmol.g-1 has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point. -1 has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka- Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. On other aspect, the intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm. The red emission was attributed to anthocyanins. Blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of petal extracts, they should possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or other. Future further investigations should clarify this point.