INVESTIGADORES
LAGORIO MarÍa Gabriela
congresos y reuniones científicas
Título:
Non-destructive assessment of water and pigments in leaves from the remission function using the Kubelka-Munk theory
Autor/es:
CORDON, G. B.; GISMONDI, S.; NIEVAS, A. V.; LAGORIO, M. G.
Lugar:
Mar del Plata
Reunión:
Congreso; AIC 2010. Interim of the International Color Association; 2010
Institución organizadora:
Grupo Argentino del color
Resumen:
Correlations between light reflectance of leaves and their pigments content are relevant in
developing non-destructive methods that allow remote sensing of vegetation. In the present
work, the applicability of the Kubelka-Munk theory and the Pile of Plates model in plant leaves
was confirmed and it was further used to elaborate a methodology for the determination of their
pigments and water concentration. Several leaves from different species: Hedera helix,Hedera helix,
Liquidambar styraciflua, Populus alba, Rosa sp., Gardenia jasminoides, Schefflera arboricola,, Populus alba, Rosa sp., Gardenia jasminoides, Schefflera arboricola,
Aloysia triphylla and Ficus benjamina were selected. Chlorophyll-a and chlorophyll-b were
quantitatively determined in the leaves by solvent extraction and subsequent spectrophotometric
determination. On the other hand, reflectance and transmittance spectra were recorded for the
studied species in the UV-visible range and in the near infra-red region. The remission function,
a quantity proportional to the chromophore concentration in solid samples, was calculated from
reflectance measurements using the Kubelka-Munk theory. Optical parameters of leaves such as
the light absorption (k) and the light scattering (s) coefficients were also estimated from
transmittance and reflectance data using the Pile of Plates model. The correlations developed in
this study allow the direct determination of chlorophylls and water in intact leaves by measuring
reflectance and transmittance spectra.
and Ficus benjamina were selected. Chlorophyll-a and chlorophyll-b were
quantitatively determined in the leaves by solvent extraction and subsequent spectrophotometric
determination. On the other hand, reflectance and transmittance spectra were recorded for the
studied species in the UV-visible range and in the near infra-red region. The remission function,
a quantity proportional to the chromophore concentration in solid samples, was calculated from
reflectance measurements using the Kubelka-Munk theory. Optical parameters of leaves such as
the light absorption (k) and the light scattering (s) coefficients were also estimated from
transmittance and reflectance data using the Pile of Plates model. The correlations developed in
this study allow the direct determination of chlorophylls and water in intact leaves by measuring
reflectance and transmittance spectra.