Induction of sporulation of cercosporoid pathogens of moth vine (Araujia hortorum

RAMÍREZ GH; ANDERSON FE; BIANCHINOTTI, MV

New Zealand Journal of Botany

RSNZ PUBLISHING

Lugar: Auckland; Año: 2019

0028-825X

The South American vine Araujia hortorum (Apocynaceae) is nowconsidered an invasive species in many countries. In New Zealandit has been targeted for biological control. In its native range inArgentina it is frequently affected by cercosporoid fungi but littleis known about the diseases they cause. These pathogensproduce no, or scarce, conidia on common artificial media thushindering certain experimental studies such as pathogenicity andhost range tests. We developed a technique to obtain abundantsporulation of two cercosporoid species isolated from diseasedleaves of this host. Sporulation was studied and compared onseveral artificial culture media after different incubation periods.The best results were obtained on a medium prepared with adecoction of the leaves of the host and the addition of CaCO3,after an incubation period of eight days at 25°C for Cercospora sp.and after five days at 20°C for Pseudocercospora fumosa. It isexpected the new medium may prove useful for othercercosporoid pathogens as well, at least for those affecting relatedhosts in the Apocynaceae.