PROFILING THE HDAC INHIBITORS ACTIVITY AGAINST THE MODEL OF CESTODE PARASITES Mesocestoides corti

VACA, HUGO R.; CELENTANO, ANA M.; MACCHIAROLI, N.; CAMICIA, FEDERICO; ROSENZVIT, MARA

Mar del Plata

Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9; 2019

Echinococcosis and cysticercosis are neglected diseases caused by the cestode parasites Echinococcus ssp. and Taenia solium, respectively. These diseases affect socioeconomically disadvantaged population and represent a significant problem in human and animal health1. There is a scarce availability of compounds approved for chemotherapy. Thus, it is imperative to identify new drugs. In this work, we investigated histones deacetylase (HDAC) enzymes as potential drug targets to develop new therapies against neglected diseases caused by cestodes. We previously reported the presence and expression of several HDAC coding genesin species from Echinococcus, Taenia, Hymenolepis and Mesocestoides genera2. Furthermore, we showed that Trichostatin A (TSA), a pan-HDAC inhibitor, decreases the parasite viability and produces alterations on the tegument and morphology and an increment of the total amount of acetylated proteins, including acetylated histone H4, on the cestode laboratory model Mesocestoides corti2. Here, we present the activity profile of a series of HDACinhibitors(HDACi) against M. corti larvae (TTy: Tetrathyridium), using a worm tracker device for high-throughput screening in parallel with microscopy observation. We evaluated a total of 40 compounds, comprising HDACi against class I, II and III HDACs.