INVESTIGADORES
MACCHIAROLI Natalia
congresos y reuniones científicas
Título:
Cestode Parasites Secrete Extracellular Vesicles Carrying Antigenic Proteins And Micrornas
Autor/es:
EUGENIA ANCAROLA; ANTONIO MARCILLA; NATALIA MACCHIAROLI; MATIAS PEREZ; SEBASTIAN ASURMENDI; CAROLINA PONCINI; MARA ROSENZVIT; MARCELA CUCHER
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta 2016 SAIC-SAI-SAFE; 2016
Resumen:
Cestode parasites are platyhelminths passively transmittedbetween the hosts involved in their life cyclesand can infect almost all vertebrate species. Some ofthe zoonoses they cause are among the most severeneglected tropical diseases in humans prioritized bythe World Health Organization. Lately, several studiesdescribed the secretion of extracellular vesicles (EV) asa path of intercellular communication in many organismsand also as a new mechanism of inter-species cross-talkin the host-parasite interplay. The term EV groups varyingtypes of membranous structures which mainly differ intheir biogenesis, morphology and protein content. EV canalso carry lipids and nucleic acids, including DNA, mRNAsand small RNAs. It has been shown that nematode andtrematode parasites secrete EV, which can be internalizedby host cells. These EV contain proteins and small RNAs,among 􀁙hich micro􀀴􀀰As 􀁙ere identified. Here, 􀁙e aimedto determine whether cestode parasites secrete EV andcharacterize their content. For this, we chose the larvalstages of the model cestodes Taenia crassiceps andMesocestoides corti. First, we demonstrated the in vitrosecretion of membranous structures compatible with EV bytransmission electron microscopy. Then, we characterizedtheir protein content by LC􀀏􀀯S/􀀯S. As a result 􀁙e identifiedexpected eukaryotic EV markers and also, among others,proteins tested for immunodiagnosis of cestode infectionas well as host immunoglobulins. Finally, we proved bycapillary electrophoresis that cestode EV carry small RNAsand then microRNAs were detected by RT-(q)PCR. Thisis the first report of E􀀸 as 􀁙ell as micro􀀴􀀰As secretion incestode parasites and could represent a new cross-speciescommunication mechanism with the host. We also provideevidence on a new route used by cestode parasites forthe secretion of formerly studied proteins. These resultsprovide relevant information for the improvement or developmentof new diagnosis methods of cestodiases.