INVESTIGADORES
MACCHIAROLI Natalia
congresos y reuniones científicas
Título:
microRNA expression profile in the metacestode stage of the human parasite Echinococcus granulosus
Autor/es:
NATALIA MACCHIAROLI; LAURA KAMENETZKY; MARCELA CUCHER; MARA ROSENZVIT
Lugar:
Rosario
Reunión:
Conferencia; 4ta. Conferencia Internacional de la Sociedad Iberoamericana de Bioinformática (SolBio). 4to. Congreso Argentino de Bioinformática y Biología Computacional (4CAB2C).; 2013
Resumen:
microRNA expression profile in the metacestode stage of the human parasite Echinococcus granulosus Natalia Macchiaroli1, Laura Kamenetzky1, Marcela Cucher1, Mara Rosenzvit1 1IMPaM, UBA-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Argentina microRNA (miRNAs) are a class of small (~22 nucleotides), non-coding RNAs that are key regulators of gene expression at post transcriptional level and play essential roles in important biological processes in animals, plants and viruses. Here we describe the microRNA expression profile in the metacestode stage of an important human parasite, Echinococcus granulosus, using Illumina deep sequencing combined with bioinformatic analysis and stem-loop reverse transcription polymerase chain reaction. Deep sequencing of two small RNA libraries prepared from two biological replicates of metacestode stage of E. granulosus produced 17.85 and 19.95 million raw reads. After low quality reads filtering, adapter trimming and read collapsing, 633,445 and 599,373 unique sequences were retained from each library respectively. Comparative analysis of the metacestode unique sequences to Echinococcus spp. miRNA sequences from protoscolex stage deposited in the miRBase showed that almost the full miRNA repertoire was present, including Echinococcus spp. specific miRNAs, with the exception of miR-7 and miR-1 suggesting that these miRNAs could be specific of the protoscolex stage. Interestingly, miR-10 and let-7, two highly conserved miRNAs across metazoans, were the most abundantly expressed; followed by miR-4989, an Echinococcus spp. specific miRNA, and then by miR-71, a miRNA conserved only across invertebrate species. Selected miRNAs were validated by stem-loop reverse transcription polymerase chain reaction in two developmental stages of the parasite: metacestode and protoscolex. Candidate novel miRNAs, additional conserved ones and other small RNA species are under analysis with available tools for non-coding RNA discovery, profiling and functional annotation based on high-throughput sequencing. This data will provide valuable information for comparative analysis with the related species E. multilocularis, the causative agent of hydatid disease in the north hemisphere.