HYBRID BACTERIAL CELLULOSE - PECTIN FILMS FOR TRANSDERMAL DELIVERY OF BIOACTIVE MOLECULES

CACICEDO ML; ISLAN GA; DRACHEMBERG F; ALVAREZ VA; BARTEL LC; BOLZAN AD; CASTRO GR

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

Novelbiopolymeric films based on bacterial cellulose (BC) modified with highmethoxylated pectin (HMP) were developed for transdermal drug delivery. Theability of films to incorporate an antibiotic, levofloxacin (Levo), wasanalyzed. Incorporation efficiencies (EE) were determined using films withdifferent proportions of HMP (from 0.1% to 2.0%) with a maximum drug payload of6.23 mg/g. Characterization studies revealed the existence of a cooperativenetwork between both polymers and deep structural changes in BC matrix.Besides, HMP presence decreased water loss in the BC film from 93% to 75% after90 min. Additionally, film incorporation capacity ofmacromolecules, using Human Serum Albumin (HSA) as a model protein, wasstudied. HMP presence enhanced in more than 3.5 times the EE of HSA and no pHdependence was observed. Release kinetics of both molecules showed hyperbolicprofiles with sustained release. On independent experiments, HMP presencegenerated around 50% decrease on both macromolecules release rates.Additionally, the incorporation of HSA into BC-HMP matrix exhibitied amodulation on Levo release profile. The antimicrobial activity of Levo releasedfrom the BC-HMP-HSA films was confirmed using Staphylococcus aureus. In-vitrostudies revealed no apparent cytotoxicity of the released compounds inmammalian CHO cells. As a conclusion, on this work the versatility of bacterialcellulose material was tested by in situmodification with an additive biopolymer. The hybrid material exhibited propercharacteristic for its application as a transdermal graft with antibioticproperties. Funding:The present work was supportedby Consejo Nacional deInvestigaciones Científicas y Técnicas (CONICET, PIP 0498), UniversidadNacional de La Plata (Grant X545, I159) and Agencia Nacional de PromociónCientífica y Técnica (ANPCyT, PICT2011-2116) of Argentina.LC Bartel had a return fellowship ofthe Alexander von Humboldt Foundation. Also, we want tothank CPKelco (Buenos Aires, Argentina) for the pectin samples.