Trypanosoma cruzi modulates LPS maturation process of DC in vitro.

PONCINI C; ALBA SOTO C; SOLANA M; BATALLA E; GONZÁLEZ CAPPA SM

Río de Janeiro, Brasil

Congreso; 13th International Congress of Immunology; 2007

International Union of Immunology Societies

Previously we reported the effects of trypomastigotes (Tp) of a virulent strain (RA) on APC, in a murine infection model. To better understand mechanisms responsible of these alterations, in vitro cocultures of bone marrow dendritic cells (BMDC) with Tp were carried out. Infection but not apoptosis was detected. Maturation phenotypes after treating these cells with LPS, Tp and Tp+LPS were studied. By flow cytometry, we analyzed the surface expression profiles of MHCII, costimulatory molecules and dextran uptake. By ELISA, we characterized secretion of pro- anti- inflammatory cytokines. Tp failed to increase MHCII expression and inhibited that induced by LPS (MFI±SE, C: 119±27, LPS: 186±40*, Tp: 111±22, Tp+LPS: 131±25, n=11, *p