INVESTIGADORES
LAROCCA Luciana
congresos y reuniones científicas
Título:
Identification and characterization of the Trypanosoma cruzi Pantothenate Transporter (TcPPT1) and its role in survival under stress conditions
Autor/es:
MD, RUIZ; L. FRACCAROLI,; BALCAZAR, D; L. LAROCCA; TORRES, PS; CARRILLO C
Reunión:
Congreso; 18th International Congress on Infectious Diseases & XVIII Congreso SADI; 2018
Resumen:
T. cruzi epimastigotes proliferation rate was significantly decreased in culture media with low concentration of pantothenate. This effect was reverted, in a concentration dependent manner, when the vitamin was added to the media. The pantothenate proliferative effect evidenced on T. cruzi epimastigotes was not observed in other axenic cultures of related trypanosomatids (T. brucei, L. mexicana and C. fasciculata). TcPPT1 overexpression was assessed by PCR, RT-PCR and fluorescence microscopy in mutant Y-GFP clones. Proliferation rate of TcPPT1-mCherry epimastigotes was significantly higher than mCherry epimastigotes (control) in low pantothenate conditions (p