Gills CYP1A of Oncorhynchus mykiss as a sensitive biomarker of crude oil pollution in freshwater environments

LEGGIERI LEONARDO RAMÓN; DE ANNA JULIETA SOLEDAD; JUAN G. CÁRCAMO; CERON GERARDO; LUIS ARIAS DARRAZ; PANEBIANCO ANTONELLA; LUQUET CARLOS MARCELO

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019 vol. 67 p. 61 - 65

1382-6689

The induction of CYP1A activity (EROD) and protein expressionwas compared in liver and gills of rainbow trout from a stream pollutedwith crude oil, and through laboratory exposures to 1% and 5% of wateraccommodated fraction of the crude oil (WAF) for 1 and 4 days. Gills ERODincreased 1.6-2.7-fold in fish from the polluted stream and duringexperiments, while liver EROD was induced only by 1% WAF at day 1 (1.5-fold). Contrastingly, crude oil pollution strongly induced both liver andgills CYP1A protein expression in the field (14-36-fold) and inexperiments (4-25-fold). This highlights that crude oil induced CYP1Aactivity markedly in gills but only slightly or not at all in the liver,suggesting that differences between organ EROD activities are related tothe modulation of CYP1A enzyme activity but not to the regulation attranscriptional or translational levels.