INVESTIGADORES
CARNEVALE MatÍas Emanuel
congresos y reuniones científicas
Título:
Is the Bes- Gluconate-Polyethylene Glycol (BGP-CS) solution suitable for the preservation of isolated hepatocytes and whole livers?
Autor/es:
CARNEVALE M.E.; TOMATIS C.; OSORIO J.S.; GUIBERT E.E.; RODRIGUEZ J.V.
Lugar:
João Pessoa
Reunión:
Congreso; X Congresso Latino Americano de Orgãos Artificiais e Biomateriais (10° COLAOB); 2018
Institución organizadora:
Sociedade Latino Americana de Biomateriais, Orgãos Artificiais e Engenharia de Tecidos - SLABO
Resumen:
We have previously described extended applications for BGP-HMP solution, initially conceived for hypothermic perfusion of whole organs. A subsequent modification in the PEG concentration turns it into in a more versatile preservation solution, renamed BGP-CS solution for static preservation. In this work we investigated its potential application in different systems: whole livers and isolated rat hepatocytes.Isolated rat liver cells were incubated in Krebs-Heinseleit solution (control) or preserved during 24 h in BGP-CS at 5°C. Both groups were immediately evaluated and after 1 h of rewarming at 37°C. Membrane integrity was studied by Trypan blue exclusion (TBE) and propidium iodide/Hoechst (Pi/H) staining. Cellular metabolism was studied by cleavage of fluorescein diacetate (FDA)-Pi, MTT metabolization and oxygen consumption. Whole rat livers were preserved in BGP-CS up to 24 h at 5°C and evaluated in an ex vivo perfusion system and their results were compared with fresh control livers, which were immediately reperfused 90 min at 37°C in the same system. Portal pressure (PF), flow rate (FR), hepatic enzyme releases, O2 consumption rate, glucose, urea production and bile flow (BF) were measured and the intrahepatic resistance (IR) was calculated.Viability and functionality studies after 24 h of hypothermic preservation of hepatocytes with BGP-CS show similar results to the control (N=8, p>0.05). Preserved livers did not show statistically differences in hemodynamically and metabolic parameters (PF, FR, IR, BF and oxygen consumption) after 90 min of reperfusion. Glycogen reserves and enzyme release were higher than controls (p
