Evaluation of a new solution for Hypothermic Machine Perfusion (HMP) of the liver. II - A study in the perfused rat liver in vitro.

CARNEVALE M.E.; BALABAN C.L.; GUIBERT E.E.; BOTTAI H.; RODRIGUEZ J.V.

Rosario

Congreso; 49th meeting of the Society for Cryobiology; 2012

Society for Cryobiology / Centro Binacional (Argentina - Italia) de Investigaciones en Criobiología Clínica y Aplicada

The present study was undertaken to develop and investigate the possible benefit of a new solution for hypothermic machine perfusion (HMP) of livers for transplants. The portal vein and bile duct of Wistar rats were cannulated, the liver excised and connected on a recirculating perfusion system with 250 mL of BES-Gluconate-Polyethylene Glycol based solution (BGP-HMP) or with Bretschneider solution (HTK) at 5 C. HMP was performed up to 24h at a constant pressure of 40 mmH2O (equivalent to 25 % of the normothermic portal pressure). Both perfusion solutions were air equilibrated during the HMP. After 24h of HMP, the livers were reperfused at 37C with Krebs-Henseleit Dextran solution (Balaban CL, Artif Organs 35(5):508-15, 2011). In both procedures, portal pressure and flow rate were measured and the intrahepatic resistance (IR) was calculated. Perfusate pH oscillations and enzymes (LDH, AST, and ALT) activities were evaluated during HMP and normothermic reperfusion. Also, the O2 consumption, glucose, urea production and bile flow were measured during the normothermic reperfusion period. Liver perfusion variables and injury parameters during HMP: Statistical differences in enzymes release (p