INVESTIGADORES
CID Mariana Paula
artículos
Título:
Phosphatidylinositol 4,5-Bisphosphate induced flunitrazepam sensitive-GABAA receptor increase in synaptosomes from chick forebrain
Autor/es:
CID MARIANA PAULA; SALVATIERRA NANCY ALICIA; ARCE AUGUSTO
Revista:
NEUROCHEMICAL RESEARCH
Editorial:
Kluwer Academic/Plenum Publishers
Referencias:
Lugar: New York; Año: 2007 vol. 32 p. 1011 - 1015
ISSN:
0364-3190
Resumen:
Abstract The flunitrazepam sensitive-GABAA receptor
density was increased by cytochalasins C and D at
37C suggesting that microfilament depolymerization
induces exposure to the radioligand of a GABAAThe flunitrazepam sensitive-GABAA receptor
density was increased by cytochalasins C and D at
37C suggesting that microfilament depolymerization
induces exposure to the radioligand of a GABAAA
receptor in synaptosomes (Pharm Biochem Behav 72
(2002) 497). Similarly, phosphatidylinositol-4,5-bisphosphate
(15 lM), but not a mixture of phospholipids,
induced an increase of GABAA receptors in
synaptosomes. Furthermore, N-ethyl maleimide, an
inactivator of the sensitive fusion protein, which
interacts with GABAA receptor, abolished the receptor
increase induced by phosphatidylinositol-4,5-bisphosphate.
Together, the results suggest that
phosphatidylinositol-4,5-bisphosphate, acts via microfilament
depolymerization increasing the binding of
the radioligand to receptors possibly by modulation of
their interaction with proteins involved in trafficking
and docking mechanisms.lM), but not a mixture of phospholipids,
induced an increase of GABAA receptors in
synaptosomes. Furthermore, N-ethyl maleimide, an
inactivator of the sensitive fusion protein, which
interacts with GABAA receptor, abolished the receptor
increase induced by phosphatidylinositol-4,5-bisphosphate.
Together, the results suggest that
phosphatidylinositol-4,5-bisphosphate, acts via microfilament
depolymerization increasing the binding of
the radioligand to receptors possibly by modulation of
their interaction with proteins involved in trafficking
and docking mechanisms.A receptors in
synaptosomes. Furthermore, N-ethyl maleimide, an
inactivator of the sensitive fusion protein, which
interacts with GABAA receptor, abolished the receptor
increase induced by phosphatidylinositol-4,5-bisphosphate.
Together, the results suggest that
phosphatidylinositol-4,5-bisphosphate, acts via microfilament
depolymerization increasing the binding of
the radioligand to receptors possibly by modulation of
their interaction with proteins involved in trafficking
and docking mechanisms.A receptor, abolished the receptor
increase induced by phosphatidylinositol-4,5-bisphosphate.
Together, the results suggest that
phosphatidylinositol-4,5-bisphosphate, acts via microfilament
depolymerization increasing the binding of
the radioligand to receptors possibly by modulation of
their interaction with proteins involved in trafficking
and docking mechanisms.