INVESTIGADORES
MENACHO MÁRQUEZ Mauricio Ariel
congresos y reuniones científicas
Título:
Key roles of Vav2 Catalysis-driven pathobiological programs in squamous cell carcinoma
Autor/es:
LUIS FRANCISCO LORENZO MARTÍN; MAURICIO MENACHO MÁRQUEZ; ABAD, ANTONIO; PARAMIO, JM; XOSÉ R. BUSTELO
Lugar:
Palm Beach
Reunión:
Congreso; Regulation and Function of Small GTPases; 2017
Resumen:
Rho GDP/GTP exchange factors (GEFs) promote the activation of Rho GTPases during signal transduction. Due to this, it is widely assumed that they play proactive roles during tumorigenesis. However, given their large numbers and multidomain structures, it is still unclear whether this idea applies to all Rho GEFs. The negative side effects that the inhibition of GEFs elicits in healthy tissues are also poorly characterized. To address those issues in the case of the Vav2 GEF, we have utilized three overlapping mouse models: (a) A ?gain-of-function? knock-in strain in which the endogenous Vav2 protein is replaced by a constitutively active counterpart. (b) A standard, ?loss-of-function? knockout strain. (c) A ?pharmaco-mimetic? knock-in strain in which wild type Vav2 is replaced by a point mutant that shows reduced catalytic activity. With such models, here we provide the characterization of a Vav2 catalysis-driven program with a prominent role in squamous cell carcinoma.