INVESTIGADORES
MENACHO MÁRQUEZ Mauricio Ariel
congresos y reuniones científicas
Título:
IN VIVO STUDIES OF THE EFFECT OF ALPHA-SYNUCLEIN INCORPORATION ON MELANOMA DEVELOPMENT
Autor/es:
FLORENCIA MALIZIA; LUCÍA ZANOTTI; GUSTAVO CHAPO; MAURICIO MENACHO MÁRQUEZ
Reunión:
Congreso; II Reunión Científica Internacional, VII Reunión Científica Regional, VI Congreso Nacional de Ciencia y Tecnología de Animales de Laboratorio; 2021
Resumen:
Synucleins are a group of neuronal proteins involved in neurodegeneration and cancer. Alpha-synuclein (αS)is the main component of Lewy bodies, the neuropathological hallmark of Parkinson's disease (PD). Themechanisms underlying aggregation, neurotoxicity, and cell-to-cell transmission of αS were explored indepth in this context. Recent evidence suggests that αS plays a role in the pathogenesis of melanoma, themost dangerous form of skin cancer, and a protective role was assigned to this protein in advancedmelanoma. However, its role in this type of cancer has not yet been fully explored. Previous results of ourgroup carried out in vitro with melanoma cells showed that they are able to incorporate different aggregationspecies of αS. These species, not only are non-toxic, but promoted instead proliferation, migration andclonogenic capacity of melanoma cells. In this work, we evaluated in in vivo models the effect of theincorporation of αS fibers on melanoma progression (CICUAL “Characterization of the role of synucleins indifferent cell types: exploring the relationship between cancer and neurodegenerative diseases”). To explorethe impact of αS incorporation on melanoma development, B16-F10 cells (control and previously incubatedwith αS fibers) were injected subcutaneously below their minimal tumorigenic dose in the right flank in 8-week-old female C57BL/6 mice. (7x104cells; n = 5/group). Using this approach, we observed that animalsinjected with pre-treated cells developed tumors within 4 weeks post-inoculation, while no tumordevelopment was observed in control animals at this time. To confirm the role of αS in melanoma growth,2x105cells (n = 6/group) were injected as described before. Tumor volume was periodically measured witha caliper during 3 weeks. Growth kinetics indicated that αS treatment significantly promoted tumor growth(P