NF-Y TFs as Key Regulators of Nodule Development and Infection

ARNAUD BETHENCOURT; LEPAGE, AGNES; REGAD, FARID; RÍPODAS, CAROLINA; BAUDIN, MAËL; FRANCE, LISA; GAMAS, PASCAL; FERNANDA DE CARVALHO-NIEBEL; NIEBEL, ANDREAS

Budapest

Conferencia; European Nitrogen Fixation Conference 2016; 2016

Plants belonging to the legume family are able to interact symbiotically with nitrogen fixing bacterianamed rhizobia. This symbiotic interaction leads to the formation of a new organ on the roots of thehost plants, called the root nodule, inside which atmospheric nitrogen is fixed for the benefit of theplant. Evidence for a key role for NF-Y transcription factors (TFs) during symbiotic infection andlegume nodule development has gradually emerged (Combier et al., 2006; Zanetti et al., 2010; Soyanoet al., 2013; Laloum et al., 2014; Laporte et al., 2014; Xiao et al., 2014; Baudin et al., 2015). NF-Y areCCAAT-box binding TFs also called nuclear factor Y (NF-Y) (Laloum et al., 2013). The heterotrimericNF-Y is composed of the DNA-binding subunit NF-YA associated with two histone-like subunits NFYBand NF-YC. We are trying to understand the mechanisms by which NF-Y TFs control noduledevelopment and infection. We have shown that MtNFYA1, MtNF-YB16, and MtNF-YC2, form themain NF-Y trimer, active during nodulation in Medicago truncatula.We will present data on the identification and characterization of their target genes using ChIP-seqand RNA-seq and also their interacting proteins partners.