INVESTIGADORES
GRAS Diana Ester
artículos
Título:
Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots
Autor/es:
ALVAREZ, JOSÉ M.; MOYANO, TOMÁS C.; ZHANG, TAO; GRAS, DIANA E.; HERRERA, FRANCISCO J.; ARAUS, VIVIANA; OBRIEN, JOSÉ A.; CARRILLO, LAURA; MEDINA, JOAQUÍN; VICENTE-CARBAJOSA, JESÚS; JIANG, JIMING; GUTIÉRREZ, RODRIGO A.
Revista:
MOLECULAR PLANT
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2019
ISSN:
1674-2052
Resumen:
Transcriptional regulation, determined by chromatin structure and regulatory elements interacting at promoter regions, is a key step in plant responses to environmental cues. Nitrate (NO3-) is a nutrient signal that regulates the expression of hundreds of genes in Arabidopsis thaliana. Here we integrate mRNA-seq, genome-wide RNA polymerase II (RNPII), ChIP-Seq and DNase-seq data sets to establish the relationship between RNPII occupancy and chromatin accessibility in response to NO3- treatments in Arabidopsis root organs. Genomic footprinting allowed us to identify in vivo regulatory elements controlling gene expression in response to NO3- treatments. NO3--modulated TF footprints are important for a rapid increase in RNPII occupancy and transcript accumulation over time. We mapped key TF regulatory interactions and functionally validated the role of NAP, a NAC-domain containing TF, as a new regulatory factor in NO3- transport. Our strategy provides a comprehensive view of transcriptional networks in response to a nutrient signal in Arabidopsis roots.