INVESTIGADORES
GRAS Diana Ester
artículos
Título:
Cytogenetic polymorphism in Prochilodus lineatus (Pisces: Characiformes) from the middle Parana River, Santa Fe City, Argentina
Autor/es:
GRAS DE; BRASSESCO MS; MARKARIANI R; RONCATI HA; SAKAMOTO-HOJO ET; FENOCCHIO AS; PASTORI MC
Revista:
COMPARATIVE CYTOGENETICS
Editorial:
ZOOLOGICAL INST
Referencias:
Año: 2007 p. 113 - 119
ISSN:
1993-0771
Resumen:
The species of the Prochilodontidae family have a stable karyotype, made of 54 biarmed chromosomes, and one pair bearing the nucleolus organizer regions (NORs). Despite its considerable economic importance in fluvial basins, no cytogenetic data are available for the Argentinian populations of the species Prochilodus lineatus (Valenciennes, 1847). Forty five P. lineatus specimens from the middle Paraná River (Santa Fe City, Argentina) were analyzed cytogenetically. Chromosome preparations were obtained by kidney cell suspension analyzed directly or after short-term culture, and processed for Giemsa staining, C- and NOR-banding. Fluorescence in situ hybridization with a 28S rDNA probe was also performed. All specimens exhibited the expected familiar karyotypic features, with 2n = 54 and fundamental number of chromosomes (FN) = 108, and C-banding pattern. Silver staining and FISH detected the NORs on a single chromosome pair. In spite of this invariable condition, positional differences on the NORs-bearing-chromosomes were observed. Seventy percent of the specimens showed interstitial NORs, while the remaining 30% presented telomeric positive bands.