In vivo response of Osteoblast-like and Odontoblast-like cells in intraperioneal diffusion chamber

RODRIGUEZ AP, TSUJIGIWA H, NAGATSUKA H, ICHIKAWA K, MINOSHINI A, MISSANA L, QIN CL, NAGAI N

Okayama (Japan)

Simposio; International Symposium of Maxillofacial & Oral Regenerative Biology; 2005

Okayama University (Japan)

Preliminary analysis of cells before using them in tissue engineering is mandatory. Thus, to clarify the behavior of cells in an appropriate experimental model, we evaluated the characteristic of MDPC-23 cells and LUSA/A1 cells in vitro and in vivo seeded in diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 dells were odontoblast-like cells but with low ability to induce dentin formation. This study suggests that MDPC-23 cells are special cells, which possess morphological and functional character of odontoblast-like cells, expressing DSPP only in vivo with capacity to induce mineralized dentin matrix