INVESTIGADORES
RODRIGUEZ Andrea Paola
congresos y reuniones científicas
Título:
Osteoblast-like and odontoblast-like cell response in vitro and rat bone activity in vivo: Effect of F-substituted apatites
Autor/es:
MIHO INOUE, RACQUEL Z. LEGEROS, HITOSHI NAGATSUKA, MASAHISA INOUE, HIDETSUGU TSUJIGIWA, ANDREA P. RODRIGUEZ, NORIYUKI NAGAI
Lugar:
Manila, Filipinas
Reunión:
Simposio; Apatites and Correlative Biomaterials; 2008
Institución organizadora:
New York University College of Dentistry
Resumen:
Different types of calcium phosphate compounds are commercially available for medical and dental applications as bone substitute materials. Biological apatites have several kinds of minor elements such as carbonate (CO3), magnesium (Mg), and fluoride (F) in enamel, dentin and bone. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells and the in vivo rat tibia activity on substituted (F-substituted) apatites (FAp).  The MC3T3-E1 and MDPC23 cells were seeded on pellets made from calcium deficient apatite (CDA), and FAp (with low, medium and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell proliferation and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. Materials were implanted in rat tibia for 1 and 2 weeks. Implanted tissues were embedded in paraffin blocks, stained with hematoxylin-eosin and histomorphometrically observed. Results showed that low F concentration induced better and faster new bone formation in vivo compared to CDA. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type and biomaterial composition and F as minor element in bone has suitable effect in bone formation in vivo