INVESTIGADORES
RODRIGUEZ Andrea Paola
congresos y reuniones científicas
Título:
Gene Expresion of Matrix Proteins in Cbfa1-knockout Mice
Autor/es:
SILVIA S. BORKOSKY; HIDETSUGU TSUJIGIWA; YOU-JIN LEE; ANDREA P. RODRIGUEZ; ROSARIO S. RIVERA; YOSHIFUMI KOMORI; HIDETSUGO NAGATSUKA
Lugar:
Kyushu
Reunión:
Congreso; 15th Annual Meeting of The Society of Hard Tissue Regenerative Biology; 2004
Resumen:
Odontogenisis and osteogenisis are two distinct yet closely related processes that share common signaling pathway including regulatory control CBbfa1. Although Cbfa1 is well recognized as the master regulated of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfa1-knockout mice at ed 17.5 and day 0 of birth, and comparing it with mandible bone development. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblast with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of ostoeblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germ: incisor tooth germ progressed to the bell stage whereas molar tooth germ showed maturational arrest to bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa1 is associated with morphogenesis of teeth and matrix protein expression, 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfa1, and 3. In Cbfa1-Knockout mice, the odontoblastic-like cells in the incisor and in spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common of teeth and bones