INVESTIGADORES
RODRIGUEZ Andrea Paola
artículos
Título:
Recombinant human bone morphogenetic protein-2/atelocollagen composite as a new material for ossicular reconstruction
Autor/es:
TAKEUCHI A; TSUJIGIWA H.; MURAKAMI J; KAWASAKI A; TAKEDA Y; FUKUSHIMA K; ANDREA P. RODRIGUEZ; HITOSHI NAGATSUKA; YAMADA M; NISHISAKI K
Revista:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
Editorial:
Wisley-Liss
Referencias:
Año: 2009 vol. 89 p. 36 - 45
ISSN:
0021-9304
Resumen:
Ossicular reconstruction is the rebuilding of the
damaged middle ear. There are many different prosthesis
and techniques used to reconstruct the middle ear ossicles.
However, precision in the surgical procedures and prostheses
used for ossiculoplasty are still imperfect. The objective
of this study was to evaluate the potential of recombinant
human bone morphogenetic protein-2 (rhBMP-2)/
atelocollagen composite for ossicular reconstruction implanted
in the tympanic cavity of rat. The ossicles were
extirpated by perforating the tympanic membranes of rats.
rhBMP-2/atelocollagen composite was implanted as substitute
of ossicles in intimate contact with the tympanic
membrane. Composites were subjected to histological, immunohistochemical,
and radiological examination. To evaluate
the auditory function, auditory brainstem response
(ABR) was measured. rhBMP-2/atelocollagen composites
showed good stability and durability without any inflammatory
reaction within the tympanic cavity. The process of
new bone formation was similar to intramembranous ossification.
They also demonstrated that the hearing ability
was re-established by ABR threshold shifts. rhBMP-2/atelocollagen
composite exhibited excellent potential for ossicular
reconstruction, maintaining their vibratory function.
This ossicular tissue engineering may be considered as a
future therapeutic strategy for ossiculoplasty