INVESTIGADORES
RODRIGUEZ Andrea Paola
artículos
Título:
Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
Autor/es:
ANDREA P. RODRIGUEZ; HIDETSUGU TSUJIGIWA; MEHMET GUNDUZ; BEYHAN CENGIZ; NORIYUKI NAGAI; RYO TAMAMURA; SILVIA S. BORKOSKY; TOHRU TAKAGI; MIHO INOUE; HITOSHI NAGATSUKA
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Año: 2009 vol. 33 p. 39 - 47
ISSN:
0327-9545
Resumen:
Progenitor cells play an important biological role in tooth and bone formation, and previousanalyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC 23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possessmorphological and functional characteristics of odontoblast-like cells expressing dentinsialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.