INVESTIGADORES
RODRIGUEZ Andrea Paola
artículos
Título:
Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells
Autor/es:
ANDREA P. RODRIGUEZ; MISSANA L; NAGATSUKA H; GUNGUZ M; TSUJIGIWA H; RIVERA R; NAGAI N
Revista:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
Editorial:
Wisley InterScience
Referencias:
Lugar: Cleveland; Año: 2006 vol. 77 p. 707 - 717
ISSN:
0021-9304
Resumen:
To induce new bone formation, mesenchymal
stem cells were seeded onto atelocollagen honeycomb scaffold.
We evaluated the efficacy of this scaffold combined
with KUSA/A1 cells in vivo. KUSA/A1 cells alone and with
atelocollagen were implanted in the subcutaneous pockets
of 4-week-old male SCID mice. The transplants were subjected
to radiographical, histological, and immunohistochemical
examinations after 2 and 4 weeks of implantation.
Radiographically, both KUSA/A1 cells alone and KUSA/
A1-atelocollagen showed some radiopaque areas formation
but the latter disclosed a larger amount. Histologically,
KUSA/A1 cells alone showed few small islands of new bone
formation surrounded by a thin layer of cellular proliferation.
On the other hand, KUSA/A1-atelocollagen revealed
abundant new bone formation as well as cellular proliferation.
We also determined the immunolocalization of type I
collagen, CD34, osteocalcin, osteopontin, and PCNA in this
newly formed bone. Our results indicated that collagen scaffold
plays an important role allowing vessel formation and
cell anchorage, especially through the proliferation and differentiation
process in a confined space. This study supports
tissue engineering as an alternative for treating different
target diseases, such as trauma or congenital defects, and
enhances existing therapeutic applications