Different SNPs in Fasciola hepatica P-glycoprotein from diverse Latin American populations are not associated with Triclabendazole resistance

SOLANA, MARÍA VICTORIA; DOMÍNGUEZ, MARÍA FERNANDA; SCARCELLA, SILVANA; RADIO, SANTIAGO; SMIRCICH, PABLO; FERNÁNDEZ, SILVINA; SOLANA, HUGO; TORT, JOSÉ F.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0166-6851

The use of Triclabendazole for controlling fasciolosis is compromised by increased drug resistance affecting livestock and humans. Although the mode of action of TCBZ is still unknown, putative candidates and markers of resistance have been advanced. A single nucleotide polymorphism (T687G) in F. hepatica PGP was proposed as marker of resistance in a small scale study of European susceptible and resistant flukes, but the association was not found in Australian samples. The T687G SNP was absent in more than 40 samples from 2 TCBZ-resistant and 3 susceptible isolates across Latin America here analyzed. While the American samples showed more variable SNPs than the previous ones, none of the SNPs detected showed a marked association with resistance.Analyzing the 42 kb of the FhPGP gene based on RNAseq data highlights that thevariation has been underestimated, suggesting that more detailed efforts are needed in order to identify markers of resistance.