ROBERT Maria Celeste
Effect of human oviductal in vitro secretion on human sperm DNA integrity
M. CELESTE ROBERT; ADRIANA CAILLE; CARLOS ZUMOFFEN; MARCELO CABADA; SERGIO GHERSEVICH
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS.
Lugar: New York; Año: 2008 vol. 25 p. 263 - 270
AbstractPurpose In the female genital tract spermatozoa interact withthe oviductal secretion. The aim of the study was to evaluatethe effect of conditioned media (CM) from cultures of humanoviductal tissue, on sperm DNA integrity. The effect of H2O2on sperm DNA integrity, before and after incubation undercapacitating conditions, was also investigated.Methods Motile sperm obtained from normozoospermicsemen samples were incubated (4 h or 22 h) in the presenceor absence of CM and further exposed to H2O2. DNAdamage was detected by the comet assay.Results The CMsignificantly reduced the DNA damage associatedwith sperm incubation, and also decreased the effect ofH2O2 after 4 h incubation, compared to controls. The H2O2caused a dose-dependent deleterious effect on sperm DNAintegrity both before and following 22 h of capacitation.Conclusion The oviductal tissue CM increased the stabilizationof the sperm DNA structure under culture conditions.