INVESTIGADORES
BIANCO Maria Isabel
congresos y reuniones científicas
Título:
NATIVE BACTERIUM WITH BIOCONTROL ACTIVITY AGAINST Xanthomonas spp.
Autor/es:
BIANCO MARIA ISABEL; MIELNICHUK, NATALIA; FELIPE, VERONICA; VOJNOV ADRIÁN A; YARYURA, PABLO MARCELO
Lugar:
Ciudad de Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017
Institución organizadora:
Sociedades de BioCiencias
Resumen:
Plant diseases caused by phytopathogenic species of Xanthomonasare currently controlled using copper compounds and resistanthost varieties. However, the use of chemical products has failed tocontrol plant diseases due to the appearance of resistant bacterialstrains. Moreover, these compounds pollute the environment andcan affect human and animal health. For these reasons, it is importantto find alternative strategies to manage diseases caused byXanthomonas. Nowadays, the use of beneficial microorganism forphytopathogens control is becoming common. Our aim is to find novelnative bacteria with antagonistic activity against different speciesof Xanthomonas: X. citri subsp citri, X. campestris pv. campestris,X. vesicatoria, X. albilineans and X. sacchari. Until now, we isolatedfrom tomato rhizosphere a bacterium (named TVM05) that antagonizesall the different strains of Xanthomonas considered in thisstudy. TVM05 is a Gram-positive spore-forming bacillus. The antagonisticactivity of TVM05 against Xanthomonas spp. was analyzedplating the phytopathogenic bacteria on tryptic soy agar and 30 μlof TVM05 free-cell supernatant (FCS) into 5 mm-wells. After incubationfor 48 h at 28 ºC, inhibition growth area was analyzed usingImage J32 program. Optimal activity of FCS was detected at earlystationary phase. Besides, results showed that FCS was stable afterheat treatment at 50 and 100 °C during 15 minutes (p