Chlorogenic acid bioavailability in Caenorhabditis elegans

CARRANZA A. ; SARAGUSTI A.; DI PAOLA NARANJO R.D.; GUSTAVO A. CHIABRANDO; ASIS RAMÓN

Rosario

Congreso; L Reunión Anual de la Sociendad Argentina de Investigación en Bioquímica y Biología Molecular; 2014

Tomato is one of the most consumed vegetables in the world and an important source of nutraceutical compounds. Recently, we measured the ability of tomato extracts to rescue C. elegans worms from thermal stress death. This activity was associated with the polyphenol composition of theses extracts. We concluded that chlorogenic acid (CGA) is the most important bioactive compound in contributing to the tolerance to thermal stress. The CGA activity might be explained by the effect of intact CGA or/and their biotransformed metabolites. However there is no information about the bioavailability of CGA in C. elegans, therefore, we developed a study to assess its bioavailability. To conduct this study, adult worms were exposed to active doses of CGA for 24 hours. Subsequently, CGA and its metabolites were extracted from the exposed worms and analyzed by HPLC-DAD-MS. CGA and at least four metabolites (caffeic acid, quinic acid, ferulic acid and 3-4 dihydroxyphenylacetic acid) were identified. These compounds were later assayed in C. elegans under thermal stress conditions, using equimolar biological active doses of CGA. All of them showed the ability to rescue the worms from death by thermal stress at different doses. These results reveal a CGA bioavailability mechanism in C. eleganssimilar to mammals and its biotransformed metabolites could mediate the CGA thermotolerance activity.