ENVIRONMENTAL CONCENTRATIONS OF CHLORPYRIFOS AFFECTS THE NORMAL FEMALE CYCLICITY IN RATS MODIFYING THE HISTOLOGICAL CHARACTERISTICS OF THE UTERUS

MARÍA ROSA RAMOS NIETO; MARIANELA LASAGNA; GABRIEL CAO; GLORIA ALVAREZ; MARÍA EUGENIA RODRIGUEZ GIRAULT; CLARA VENTURA; FLORENCIA CHIAPPINI; CLAUDIA COCCA; NÚÑEZ MARIEL

Mar del Platra

Congreso; LXIII REUNION CIENTIFICA DE LA SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA; 2018

Previously, we demonstrated that the pesticide chlorpyrifos (CPF) acts as an endocrine disruptor in human mammary adenocarcinoma cells MCF-7 and in rat mammary gland.We investigated whether environmental concentrations of CPF induce changes in the uterus, and whether this effect depends on the accumulation of CPF in serum or fat in rats.Female 40-day-old Sprague-Dawley rats were exposed to CPF 0,01 y 1 mg/Kg/día (CPF 0,01 y CPF 1) or vehicle (C) orally for 100 d. We determined the estrous cycle phase daily. The rats were euthanized in estrus. The uterus, abdominal fat and blood were removed. Serum and fat CPF-levels were determined by gaseous chromatography. We performed the anatomopathological study of the uterus. Immunohistochemical expression of the proliferating cell nuclear antigen (PCNA) was determined. Fractal dimension (FD) analysis, a measure of the irregularity and complexity of the tissue was used.Serum levels of CPF were 0.107 +/- 0.10 ng/mL and 0.62+/- 0.22 ng/mL in the animals of CPF 0.01 y CPF 1 groups, respectively and undetectable in group C. In fat we found a significant accumulation of the pesticide in the CPF 1 group (14.16+/-9.48 ng/gr fat; p