PERSONAL DE APOYO
CAO Gabriel Fernando
artículos
Título:
Rate of atherosclerosis progression in ApoE-/- mice long after discontinuation of cola beverage drinking
Autor/es:
OTERO-LOSADA M, CAO G, MC LOUGHLIN S, RODRÍGUEZ-GRANILLO G, OTTAVIANO G, MILEI J
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2014 p. 1 - 6
ISSN:
1932-6203
Resumen:
This study was conducted in order to evaluate the effect of cola
beverages drinking on atherosclerosisand test the hypothesis whether
cola beverages consumption at early life stages might affect the
development and progression of atherosclerosis later in life. ApoE-/-
C57BL/6J mice (8 week-old) were randomized in 3 groups (n = 20 each)
according to free accessto water (W), sucrose sweetened carbonated cola
drink(C) or aspartame-acesulfame K sweetened carbonated 'light' cola
drink (L)for the next 8 weeks. Drinking treatment was ended by switching
C and L groups to drinking water. Four mice per group and time were
sequentially euthanized: before treatment (8 weeks-old), at the end of
treatment (16 weeks-old) and after treatment discontinuation (20
weeks-old, 24 weeks-old, 30 week-old mice). Aortic roots and livers were
harvested, processed for histology and serial cross-sections were
stained. Aortic plaque area was analyzed and plaque/media-ratio was
calculated. Early consumption of cola drinks accelerated atherosclerotic
plaque progression favoring the interaction between macrophages and
myofibroblasts, without the participation of either T lymphocytes or
proliferative activity. Plaque/media-ratio varied according to drink
treatment (F2,54 = 3.433, p<0.04) and mice age (F4,54 = 5.009,
p<0.03) and was higher in C and L groups compared with age-matched W
group (p<0.05 at 16 weeks and 20 weeks, p<0.01 at 24 weeks and 30
weeks). Natural evolution of atherosclerosis in ApoE-/- mice (W group)
evidenced atherosclerosis acceleration in parallel with a rapid increase
in liver inflammation around the 20 weeks of age. Cola drinking within
the 8-16 weeks of age accelerated atherosclerosis progression in ApoE-/-
mice favoring aortic plaque enlargement (inward remodeling) over media
thinning all over the study time. Data suggest that cola drinking at
early life stages may predispose to atherosclerosis progression later in
life in ApoE-/- mice.