PERSONAL DE APOYO
CAO Gabriel Fernando
artículos
Título:
HIGH-SODIUM DIET PROMOTES A PROFIBROGENIC REACTION IN NORMAL RAT KIDNEYS: EFFECTS OF TEMPOL ADMINISTRATION
Autor/es:
ROSÓN MI, DELLA PENNA SL, CAO G, GORZALCZANY S, PANDOLFO M, CERRUDO C, FERNÁNDEZ BE, TOBLLI JE
Revista:
JN. JOURNAL OF NEPHROLOGY
Editorial:
WICHTIG EDITORE
Referencias:
Lugar: Milano; Año: 2011 p. 119 - 127
ISSN:
1121-8428
Resumen:
Background: Studies carried out in vitro have recently
shown that salt loading induces an increasing mechanical
stretch and a flow-induced superoxide production
in the thick ascending limb of Henlefs loop. In
this regard, we hypothesized that the oxidative stress
induced by salt overload could stimulate inflammatory
and fibrogenic signaling pathways in normal rats.
Methods: Sprague Dawley rats were fed with an 8%
NaCl high- (HS) or 0.4% NaCl normal-salt (NS) diet
for 3 weeks, with or without Tempol (T) administration
(1 mM, administered in drinking water). Mean arterial
pressure (MAP), glomerular filtration rate (GFR)
and urinary sodium excretion (UVNa) were measured.
NAD(P)H oxidase p47phox, angiotensin II (Ang II),
transforming growth factor À1 (TGF-À1), ¿-smooth
muscle actin (¿-SMA) and nuclear factor-kappa B (NFkB)
expression were evaluated in renal tissues by immunohistochemistry.
Results: A high NaCl diet produced a slight but significant
increase in MAP and enhanced UVNa and oxidative
stress. Administration of a high NaCl diet induced
the overexpression of TGF-À1, ¿-SMA and NF-{B in
cortex and medulla, while Ang II increased in proximal
convoluted tubules, and decreased in cortical collecting
ducts. Tempol administration prevented these
changes and simultaneously normalized MAP accompanied
by an enhancement in GFR and UVNa.
Conclusion: The results showed that a high NaCl diet
is able to produce a renal profibrotic response also in
normal rats, which could be associated with oxidative
stress rather than intrarenal Ang II expression.