INVESTIGADORES
PEREIRA Nair De Los Angeles
congresos y reuniones científicas
Título:
Alkaline Phosphatase in shrimp Artemesia longinaris : Response to vitamin D in diet.
Autor/es:
FENUCCI, J.L; PEREIRA, N.A; FERNÁNDEZ GIMENEZ, A.V. AND HARÁN, N.S
Lugar:
Natal
Reunión:
Congreso; World Aquaculture 2011; 2011
Institución organizadora:
World Aquaculture Society
Resumen:
Artemesia longinaris is an abundant penaeid species in the Argentine coastal waters. As the availability of this species undergoes yearly fluctuations, it is important to establish the feasibility of culturing. Diet quality is an important condition for a successful culture of crustaceans. Numerous studies have revealed that fat-soluble vitamins are essential in most animals for normal health and life functions. Vitamin D plays an important role in calcium metabolism, and the lack of this nutrient was correlated with a variation in alkaline phosphatase activity in crustaceans. The present study shows the occurrence of different alkaline phosphatase activities in the midgut gland and haemocytes of Artemesia longinaris in response to varying levels of Vitamin D in diet. Five semi purified diets (vitamin free casein, manioc starch, squid protein concentrate and vitamin free fish oil) were designed containing 0 (D0); 8,000 (D8), 15,000 (D15); 30,000 (D30) and 40,000 (D40) IU vitamin D3/kg diet. Individuals were obtained from a commercial fisherman and placed in 150 l glass aquaria (33? salinity, 20°C, pH 7, 13:11 h photoperiod). Each diet was tested in three replicate groups of 8 animals/m2 during 6 weeks. At the end of the trial all specimens were placed on ice and midgut glands and haemolymph were extracted. Wild organisms (Di) were used as control. Alkaline phosphatase activity was determined in the protein extract of midgut gland and haemocytes (HLS) using p-nitrophenylphosphate (p-NPP) (Sigma N4645) as substrate. Several authors reported that both the absence and levels above 0.1 mg vitamin D /kg in diet, correlates with a variation in the activity of the alkaline phosphatase in Penaeus monodon. Alkaline phosphatase activities obtained for individuals fed D8; D15 and D30 had the nearest values to those obtained for wild animals. However the other diets had higher values which can be attributed to nutritional stress. It was pointed out that alkaline phosphatase activity can be used as an indicator of biochemistry stress. The results obtained in the present study suggests that alkaline phosphatase can be use as stress bioindicator and that both, deficiency and hypervitaminosis of Vitamin D in A. longinaris increase the enzyme activity. Figures 1 and 2 show the values of phosphatase activities in hepatopancreas and HLS in response to varying levels of vitamin D in diet.